LED phototherapy in tissue repair of chronic wounds in people with diabetes: a systematic review.

OBJECTIVE: To identify scientific evidence of LED photobiomodulation in the treatment and tissue repair of chronic wounds in people with Diabetes Mellitus, types I and II. METHOD: Systematic review conducted from September/2021 to April/2022 in PubMed, LILACS, SCIELO, COHRANE, EMBASE and Web of Science. Randomized and observational clinical trials using LED in wound healing in diabetics, published between 2015 and 2022 were included. Data were descriptively analyzed with title/abstract screening, full text articles reading and definitive selection after meeting the predefined inclusion and exclusion criteria. RESULTS: Fromthe total of 840 references, eight articles were selected, that evaluated the effectiveness of LED phototherapy in wounds of diabetic patients. CONCLUSION: LED light proved to be beneficial in tissue repair, with increased production in collagen and fibroblasts, angiogenesis, reduction of inflammation and, consequently, a decrease in lesion size.

LED phototherapy in tissue repair of chronic wounds in people with diabetes: a systematic review / Fototerapia LED en la reparación de tejidos de heridas crónicas en personas con diabetes: una revisión sistemática / Fototerapia com LED no reparo tecidual de feridas crônicas em pessoas com diabetes: revisão sistemática

ABSTRACT Objective: To identify scientific evidence of LED photobiomodulation in the treatment and tissue repair of chronic wounds in people with Diabetes Mellitus, types I and II. Method: Systematic review conducted from September/2021 to April/2022 in PubMed, LILACS, SCIELO, COHRANE, EMBASE and Web of Science. Randomized and observational clinical trials using LED in wound healing in diabetics, published between 2015 and 2022 were included. Data were descriptively analyzed with title/abstract screening, full text articles reading and definitive selection after meeting the predefined inclusion and exclusion criteria. Results: Fromthe total of 840 references, eight articles were selected, that evaluated the effectiveness of LED phototherapy in wounds of diabetic patients. Conclusion: LED light proved to be beneficial in tissue repair, with increased production in collagen and fibroblasts, angiogenesis, reduction of inflammation and, consequently, a decrease in lesion size.

RESUMEN Objetivo: Identificar evidencias científicas de fotobiomodulación con LED en el tratamiento y reparación de tejidos de heridas crónicas en personas con Diabetes Mellitus, tipos I y II. Método: Revisión sistemática realizada de septiembre/2021 a abril/2022 en PubMed, LILACS, SCIELO, COHRANE, EMBASE y Web of Science. Se incluyeron ensayos clínicos aleatorizados y observacionales con uso de LED en la cicatrización de heridas en diabéticos, publicados entre 2015 y 2022. Los datos fueron analizados descriptivamente con selección de título/resumen, lectura de artículos a texto completo y selección definitiva después de cumplir con la inclusión e inclusiones ex - predefinidas. Resultados: Del total de 840 referencias encontradas, se seleccionaron ocho artículos que evaluaron la efectividad de la fototerapia LED en heridas de pacientes diabéticos. Conclusión: La luz LED demostró ser beneficiosa en la reparación de tejidos, con aumento de la producción de colágeno y fibroblastos, angiogénesis, reducción de la inflamación y, en consecuencia, disminución del tamaño de la lesión.

RESUMO Objetivo: Identificar evidências científicas da fotobiomodulação com LED no tratamento e reparo tecidual em feridas crônicas de pessoas com Diabetes Mellitus, tipo I e II. Método: Revisão sistemática realizada de setembro/2021 a abril/2022 na PubMed, LILACS, SCIELO, COHRANE, EMBASE e Web of Science. Incluídos ensaios clínicos randomizados e observacionais utilizando LED na cicatrização de feridas em diabéticos, publicados entre 2015 a 2022. Os dados foram analisados descritivamente com triagem de título/resumo, leitura dos artigos em texto completo e seleção definitiva após atender aos critérios de inclusão e exclusão pré-definidos. Resultados: Do total de 840 referências encontradas, foram selecionados oito artigos que avaliaram a eficácia da fototerapia LED em feridas de pacientes diabéticos. Conclusão: A luz LED mostrou-se benéfica no reparo tecidual, com aumento na produção de colágeno e fibroblastos, angiogênese, redução da inflamação e consequentemente, diminuição no tamanho da lesão.

Chitosan suspension as extractor and encapsulating agent of phenolics from acerola by-product.

The polymeric suspension of chitosan (Ch) has been an effective media for the extraction of total phenolic compounds (TPC) from the acerola by-product. It facilitates the subsequent production of nanoparticles loaded with the phenolics (Np-TPC) by ionic gelation. However, neither the effects of Ch concentration on encapsulation efficiency (EE%) of TPC nor which compounds are extracted in its media are known, being it the first objective of this study. The second objective was to analyze the stability of the Np-TPC under accelerated conditions and its release profile at pHs 3.0 and 7.0. The results showed that Ch does not affect the extraction of TPC. However, the EE increased from 35.0 to 48.1 % with the increase of Ch concentration (0.4 to 1.0 %). LC/ESI-QTOF MS analysis showed that phenolic acids and flavonoids are extracted in 0.8 % Ch medium. After encapsulation, microscopy images revealed particle sizes ranging between 110 and 150 nm. Additionally, the presence of phenolics did not change the stability of the particles under accelerated conditions and the actives were fully released into the released medium for 10 h. The Np-TPC suspension appears to be useful for the production of edible antioxidant coatings to preserve fruits/vegetables, with potential application as carrier of other food ingredients.

Extraction of phenolic compounds from acerola by-products using chitosan solution, encapsulation and application in extending the shelf-life of guava.

Aiming the simplification of the production of chitosan nanoparticles as an encapsulating material, the primary approach of this study was to investigate the extraction of active compounds from acerola-pulp by-products directly in chitosan solution by using tip sonication. The results have shown that chitosan solution can be used as a good solvent, mainly for total phenolic compounds (TPC) extraction (1792.7 mg/100 g of dry by-product). The extract was submitted to ionic gelation process using, as counter-ion, the sodium tripolyphosphate to form loaded nanoparticles with TPC. The suspension was applied as protective coatings on the guavas. The nanoengineered coatings provided an effective barrier that delayed the maturation and maintained the green pigmentation for longer periods along with good firmness. To the best of our knowledge, this was the first study that uses chitosan solution as extraction solvent of TPC from food byproducts in order to facilitate the encapsulation process.

Determinação de coliformes em linguiças de carne suína industrializadas comercializadas no município de Ceres, GO / Determination of coliforms in industrialized swine sausages marketed in the municipality of Ceres, GO

O consumo de produtos à base de carne suína, como a linguiça suína, vem crescendo, tornando-se necessário o controle das condições higienicossanitárias. Objetivou-se pesquisar coliformes em linguiça suína comercializada nos açougues na cidade de Ceres, GO. Foram coletadas 20 amostras, aleatoriamente, em 20 casas de carnes. As amostras foram analisadas segundo a metodologia de Silva et al. (2001) para determinação do Número Mais Provável de coliformes totais e termotolerantes. Os resultados foram comparados com a Resolução RDC nº 12, de 02 de janeiro de 2001. Na análise de coliformes totais 95% das amostras apresentaram contaminação, com valores acima de 2,4x10³ NMP/g, equivalendo a 35% das amostras analisadas. Para coliformes termotolerantes, das 20 amostras analisadas, 90% (18) apresentaram contaminação e em 25% (5) houve crescimento acima de 2,4x10³ NMP/g. Cinco das vinte amostras analisadas estavam fora dos padrões estabelecidos, pois apresentaram nível de contaminação superior ao da legislação vigente.

Determinação de coliformes em carne bovina moída comercializada em açougues da cidade de Ceres - GO / Determination of coliforms in beef marketed at butcher in the city of Ceres - GO

Objetivou-se avaliar a qualidade microbiológica de carne bovina moída comercializada nos açougues da cidade de Ceres/GO. Foram coletadas 20 amostras em cinco açougues diferentes com quatro coletas por açougue. As amostras foram analisadas para determinação do Número Mais Provável de coliformes totais e termotolerantes. Os resultados foram comparados com a legislação vigente. Na análise de coliformes totais 100% das amostras apresentaram contaminação, havendo crescimento acima de 2,4x10³ NMP/g equivalendo a 80% (16) das amostras analisadas. Para coliformes termotolerantes das 20 amostras analisadas houve crescimento acima de 2,4x10³ NMP/g em 51%. As análises microbiológicas da carne moída, quando comparadas à legislação vigente, apresentaram nível elevado de contaminação evidenciando condições higienicossanitárias deficientes.